Home > Sandwich ELISA Kits > Mouse Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2) ELISA Kit

Product Name:

Mouse Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2) ELISA Kit

Cat.#:

JL17340

Regulatory Status:

RUO

Alternative:

CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.63ng/mL

Specificity:

This kit recognizes Mouse VEGFR-2 in samples. No significant cross-reactivity or interference between Mouse VEGFR-2 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| VEGFR-2

Kinase insert domain receptor (KDR, a type IV receptor tyrosine kinase) also known as vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. KDR has also been designated as CD309 (cluster of differentiation 309). KDR is also known as Flk1 (Fetal Liver Kinase 1).The Q472H germline KDR genetic variant affects VEGFR-2 phosphorylation and has been found to associate with microvessel density in NSCLC.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.05	1.5	0.99	0.66	0.45	0.33	0.24	0.08
Corrected OD	1.97	1.42	0.91	0.58	0.37	0.25	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse VEGFR-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Mouse VEGFR-2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	88-103	87-108
1 : 4	Average (%)	94	98